重氮乳凝法与胶体金法、聚合酶链反应诊断结核病的比较

Comparism of the diazopolystyrene latex agglutination test with colloidal gold-technique and PCR for diagnosis of tuberculosis

目的 评价应用重氮乳凝试验诊断结核病的临床实用价值。方法 以胶体金法和聚合酶链反应(PCR)为对照 ,分别用重氮乳凝法和胶体金法检测 91份临床诊断为结核病的患者血清标本 ,以PCR法检测患者相应的痰标本。结果 重氮乳凝法与胶体金法和PCR比较 ,阳性符合率分别为 98 1%和 89 8% ;敏感性分别为96 3%和 72 9% ;特异性分别为 97 3%和 6 8 8%。结论 重氮乳凝法可用于结核病实验室诊断 。

Objective To evaluate the applicability of diazopolystyrene latex agglutination test (DLAT) in tuberculosis. Methods With colloidal gold technique and PCR test as control, DLAT was used to detect 91 patients with pulmonary tuberculosis. Results Compared with colloidal gold technique and PCR test and DLAT had positive coincidence rate of 98.1% and 89.8%, sensitives of 96.3% and 72.9% and specificities of 97.3% and 68.8%, respectively. Conclusion DLAT could be used for laboratory diagnosis of TB in China.