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应用NASBA定量检测HCV RNA的研究进展 被引量:11

Applying NASBA to the quantitative of the advancing research on HCV RNA
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摘要 NASBA(nucleicacidsequence basedamplification)即核酸序列依赖性扩增法 ,是由一对引物介导的、连续均一的、体外特异性核苷酸序列等温扩增的RNA新技术。反应在 42℃进行 ,可在 2h内将RNA模板扩增约 10 9倍。
出处 《热带医学杂志》 CAS 2002年第1期64-66,共3页 Journal of Tropical Medicine
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参考文献9

  • 1Hollingsworth R, Sillekens P, Van P, et al. Serum HCV RNA levels assessed by quantitive NASBA: stability of viral load over time, and lack of correlation with liver disease. J Hepatol, 1996;25:301-306
  • 2Lunel F, Mariotti M, Cresta P, et al. Comparative study of conventional and novel strateries for the detection of hepatitis C virus RNA in serum:amplicor, branched-DNA, NASBA and in-house PCR. J Virol Methods, 1995;54:159-171
  • 3Lunel F, Cresta P, Vitour D, et al. Comparative evaluation of Hepatitis C Virus RNA Quantitation by Branched DNA, NASBA,and Monitor Assays. Hepatol, 1999;29:528-535
  • 4Compton J. Nucleaic acid sequence-based amplication. Nature, 1991;350(6313): 91-92
  • 5Ppeignoux M, Marcellin P, Pouteau M, et al . Pretreatment serum hepatitis C virus RNA levels and hepatitis C virus genetype are main and independent prognostic factors of sustained response to interferon alfa therapy in chronic hepatitis C. Hepatol, 1995;22: 1050-1056
  • 6Kievits T, Van G, Van S, et al. NASBA isothermal enzymatic in vitro amplication optimized for the diagnosis of HIV-1 infection. J Virol Methods, 1991;35:273-286
  • 7Mahony J, Song X, Chong S, et al. Evaluation of the nuclisens basic kit for detection of chlamydia trachomatis and neisseria gonorrhoeae in genital tract specimens using nucleic acid sequence-based amplication of 16S r RNA. J Clin Microbial, 2001;39(4): 1429-1435
  • 8Michel P, Maaike W, Van D, et al. Single rapid real-time monitored isothermal RNA amplication assay for quanitification of human immunodeficiency virus type I isolates from groups M, N, and O. J Clin Microbial, 2001;39(4):1378-1384
  • 9Tsuruoka M, Fujii T. Rapid and specific detection of RNA base sequence using fluorescence polorization. Nucl Acids Symp Ser, 1999;42:239-240

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