高孔隙率滨珊瑚用于组织工程骨的构建

Tissue engineering bone with higher porousity coralline as scaffolds

目的 探讨西沙群岛高孔隙率滨珊瑚作为骨组织工程支架材料的可行性 ,为组织工程研究开辟新的途径 .方法 取 4 wk龄兔骨髓 ,分离骨髓基质细胞 ,体外培养 ,经诱导分化为成骨细胞 ,接种至滨珊瑚材料 ,无菌条件下植入裸鼠皮下组织中 .对照组单纯植入滨珊瑚 .分别于 4 ,8wk取材 ,行大体观察、X线摄影及组织学染色 ,观察新骨形成情况 .结果 4 wk时 X线片有高密度阻射影像 ,HE染色可见有新骨形成 ;8wk时 X线阻射影像密度更高 ,HE染色可见大量新骨形成并相互连接成骨小梁样结构 ,骨细胞位于陷窝中 .结论 高孔隙率滨珊瑚可以作为骨组织工程的支架材料并有广阔的应用前景 .

AIM To investigate the feasibility of using coral as scaffolds in bone tissue engineering. METHODS The marrow stromal cells from New Zealand rabbits of 4 months were harvested and cultured in vitro. After multiplied, dexamethasone was used to promote the osteoblastic phenotype of the cells. The cells were harvested and then seeded into coral. By means of tissue engineering, osteoblastic cells/coral complexes were formed. The complexes were implanted subcutaneously in nude mice and coral alone was implanted as the control. Bone regeneration was assessed by histological and roentgenographic analysis 4 and 8 weeks after the implantation. RESULTS Osteoid tissues were observed 4 weeks after the implantation; large amount of new bone was found 8 weeks after the implantation; lamellar bone was observed, and many osteoblasts could be seen in the bone matrix. CONCLUSION The coral with higher porous rate can be used as a good scaffold material for bone tissue engineering.