摘要
目的 :研究维生素E (VE)防治肝纤维化可能存在的作用机制。方法 :用 4 0 %CCl4皮下注射 9周制作大鼠肝纤维化模型 ,在肝纤维化形成后 ,治疗组用 5 %VE乳剂静脉注射 (10 0mg/kg ,2次 /周 )连续 10周。于治疗后 10周取各组肝组织作病理检查 ,并用原位杂交技术检测肝组织转化生长因子 β1(TGF - β1)、基质金属蛋白酶 - 1(MMP - 1)mPNA表达 ,对检测结果进行图像分析。结果 :治疗组肝脏纤维组织沉积较对照组减少 ,TGF - β1mRNA表达明显低于对照组 ,经图像分析与对照组差异有显著性 (P <0 .0 1)。MMP - 1mRNA的表达两组之间未见显著差异。结论 :维生素E可能通过下调TGF- β1mRNA表达 ,减缓大鼠肝纤维化。
Objective :To study the effects of vitamin E(VE) on liver fibrosis and its mechanism. Method: Model of liver fibrosis was induced by subcutaneous injection of CCL 4 for nine weeks in rats. The control group did not receive any treatment. The treatment group was treated with VE (100mg/kg, I.V.)twice a week for 10 weeks after the model was established. Histopathological examination was made at the 10th week after the administration of VE. At the same time, mRNA expression of transforming growth factor β1(TGF-β1)and matrix metalloproteinase-1(MMP-1) in the liver tissue were observed with in situ hybridization. Result: Histologically, liver fibrosis was severe in the rats of the control group, but improved significantly in rats of the treatment group. In situ hybridization and image analysis showed that expression of TGF-β1 mRNA decreased significantly in the treatment group. These changes were statistically significant as compared with those of the control group (P<0.01). No obvious difference was found between treatment group and control group in expression of MMP-1. Conclusion :VE could down-modulate the expression of TGF-β1 mRNA in the liver of rats with liver fibrosis. Thereby, it is suggested that it may play a role in the attenuation of fibrosis.
出处
《西南国防医药》
CAS
2002年第1期5-7,F003,共4页
Medical Journal of National Defending Forces in Southwest China
