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hTERT基因反义核酸对Hela宫颈癌细胞端粒酶活性的影响

ATTENUATION OF TELOMERASE ACTIVITY BY USING AN ANTISENSE OLIGONUCLEOTIDE AGAINST hTERT mRNA IN CERVICAL CANCER CELLS.
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摘要 目的 :研究人类端粒酶催化亚单位 (hTERT)基因的反义寡核苷酸对宫颈癌细胞的影响。方法 :采用TRAP -ELISA检测Hela细胞在反义核酸处理前后端粒酶活性的变化。结果 :hTERT基因反义核酸能有效抑制Hela细胞端粒酶活性。结论 :hTERT基因反义核酸能显著抑制肿瘤细胞的端粒酶活性 ,为肿瘤治疗提供了一个有效的新途径。 Objective: To study the effects of antisense oligonucleotide against human telomerase catalytic subunit mRNA(hTERT mRNA)on human telomerase activity in cervical cancer cells in vitro. Method :Telomerase activity of cultured cervical cancer cells(Hela cells) was measured using the TRAP-ELISA assay before and after antisense treatment. Result: The antisense treatment could effectively inhibite the telomerase activity of Hela cells. Conclusion: Antisense oligonucleotide direct against hTERT mRNA could effectively inhibite the telomerase activity of tumor cells, which show an effective and novel approach for treatment of cancers.
作者 韩风华 辛晓燕 杜辉 王健
机构地区 第四军医大学西京医院
出处 《西南国防医药》 CAS 2002年第1期7-10,共4页 Medical Journal of National Defending Forces in Southwest China
关键词 HTERT基因 反义核酸 HELA 宫颈癌 细胞 端粒酶 活性 端粒酶催化亚单位 反义治疗 telomerase, telomerase catalytic subunit, cervical carcinoma, antisense treatment
分类号 R737.33 [医药卫生—肿瘤]
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参考文献15

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西南国防医药
2002年 第1期

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