人肝癌细胞株HepG2和BEL-7402对放射敏感性的体外实验研究

Radiosensitivity of Hepatocarcinoma Cell Lines HepG2 and BEL-7402

目的 采用体外实验方法探讨人肝癌细胞株HepG 2和BEL -740 2对放射的敏感性。方法 对人肝癌细胞株HepG2和BEL -740 2进行体外培养 ,应用集落形成法和MTT法测定 6MVX线不同剂量照射后细胞的存活率 ,计算 2株细胞放射敏感性参数 (D0 )。结果 HepG 2的D0 值为 1.5 ,SF2 为 (0 .45± 0 .0 5 ) ;BEL -740 2的D0 值为 1.6,SF2 为 (0 .63± 0 .0 5 )。MTT法与集落形成法相关性较好 (γ =0 .946,P <0 .0 5 )。结论 HepG2具有较高的放射敏感性 ,BEL -740 2的放射敏感性较低 ;MTT法可替代集落形成法作细胞放射敏感性的快速测定。

Objective To evaluate the radiosensitivity of hepatocarcinoma cells HepG2 and BEL-7402 in vitro.Methods Using cell culture of human being hepatocarcinoma cell lines HepG2 and BEL-7402,we measured their surviving fraction by clonogenic assay and MTT after irradiated by 6MV X ray with doses ranging 0～10 Gy.Radiosensitivity index,D 0,was calculated.Results The D 0 of HepG2 was 1.5 Gy,SF 2(0.45±0.05).The D 0 of BEL-7402 was 1.6 Gy,SF 2(0.63±0.05).There was a good correlation between the results derived from MTT and clonogenic assay(γ=0.946, P <0.05).Conclusion The radiosensitivity of HepG2 is rather high compared with BEL-7402.MTT is a quick way to measure radiosensitivity of cells in vitro instead of clonogenic assay.