N-去硫酸肝素对肝缺血再灌注损伤的作用

Effects of N - Desulfated Heparin on Liver Ischemia and Reperfusion Injury in Rats

目的研究N-去硫酸肝素对大鼠肝缺血再灌注损伤的防治作用。方法 建立肝缺血再灌注损伤大鼠模型,用N-去硫酸肝素对其进行阻断治疗。免疫组化LSAB法检测肝组织P选择素表达。结果 缺血再灌注后大鼠肝细胞显著水肿、空泡变性、炎性细胞浸润,出现大片状坏死灶,血清丙氨酸转移酶和天冬氨酸转移酶水平明显升高（P< 0.01）,且P选择素在肝组织中表达。而再灌注前5min静脉注射N-去硫酸肝素,则仅见部分肝细胞轻度水肿、变性及少量的散在点状坏死或局灶性坏死灶伴少量的炎性细胞浸润,未见片状坏死灶,血清酶水平明显减低,肝组织未见P选择素表达。且发现N-去硫酸肝素对凝血指标（PT和KPTT）无明显影响。结论 N-去硫酸肝素对肝缺血再灌注损伤具有防治作用,但对PT和KPTT无明显影响。

To investigate the effects of N - desulfated heparin on liver ischemia and reperfusion injury. Methods Using a rat model of 60 minute liver ischemia followed by 24h reperfu-sion,animals were randomly divided into 3 groups: the sham - operated controls, ischemic group receiving only normal saline,and treated group receiving N - desulfated heparin at a dose of 12mg/kg at 5min before reperfusion. P - selectin expression was detected in liver tissues with immunohistochemistry method. Results P-selectin expression, serum ALT and AST levels were significantly increased during 60 minute left lobar ischemia and 24h reperfusion, while the increment was significantly inhibited, and liver pathology observed by light microscopy was remarkably attenuated by treatment with the N - desulfated heparin. And the heparin was found no effects on PT and KPTT. Conclusion Our results suggest that this N - desulfated heparin can be of therapeutic potential for hepatic damage induced by local ischemia and reperfusion injury without significant anticoagulant activity.