嗅神经鞘细胞在体外培养时对GABA能神经元的作用

NEUROTROPHIC EFFECT OF ENSHEATHING CELLS ON GABAERGIC NEURONS in vitro

目的 研究嗅神经鞘细胞 (OECs)在培养状态下对GABA能神经元的存活及突起生长的影响。 方法 用原代培养的方法 ,分别进行OECs和脊髓神经元的培养 ,培养 6d后 ,将OECs接种在Millipore细胞培养板的内嵌培养皿中 ,然后置入脊髓神经元的培养板中 ,使两者进行不接触的联合培养 ,培养进行至 6d ,分别作GABA免疫细胞化学染色 ,观察突起生长情况以及GABA阳性细胞计数。 结果 与OECs联合培养组 (实验组 )GABA能神经元存活为 39 7± 6 3,对照组为 2 7 6± 2 7(P <0 0 5 ) ,并且实验组GABA能神经元染色较对照组深 ,突起长度(6 30± 112 μm)较对照组长 (2 70± 97μm) (P <0 0 1)。 结论 在体外培养状态下 ,OECs对GABA能神经元有明显的促进作用。

Objective The purpose of the present study is to illustrate the effect of olfactory ensheathing cells(OECs) on the survival and neurite outgrowth of GABAergic neurons in vitro. Methods OECs were dissociated from olfactory bulb and neurons from spinal cord of E12 mouse. On the sixth day in vitro,the Millipore cultue blank with OECs was transferred to the neuron culture mediam and continue the co-culture for another 6 days.The cultured neurons were stained with anti-GABA antibody.The neurite of neurons was observed with an image system.The number of GABAergic positive neurons was counted under the microscope. Result The number of GABAergic neurons was 39^7±6^3 in co-culture groups,whereas the number of GABAergic neurons represented only 27^6±2^7 in control groups(CG),(P<0^05).The GABAergic neurons of EG showed stronger staining than CG.And the neurite length of GABAergic neurons (630±112)μm of EG was longer than CG(270±97)μm,(P<0 01).Conclusion The soluble factors derived from OCEs can significantly promote the activity of GABAergic neurons.;