CKIp15^(INK4B)相关新基因p15rs对HeLa细胞增殖影响的初探

THE EFFECT OF p15rs-A NEW GENE RELATED TO CKIp15^(INK4B) ON THE PROLIFERATION OF HeLa CELLS

目的 探讨新克隆的CKIp15 INK4B 相关基因p15rs对细胞增殖的作用。 方法 克隆p15rs编码区cDNA ,通过基因转染技术将其导入HeLa细胞 ,检测细胞的生长曲线 ,细胞周期和集落形成能力。 结果 构建了p15rs高表达的细胞模型HPS2 1,发现p15rs高表达可使HeLa细胞增殖被显著抑制 ,细胞生长速率下降 ,倍增时间延长 ,G1 期细胞增加 ,S期细胞减少 ,集落形成能力下降。 结论 新基因p15rs具有抑制癌细胞增殖和对细胞周期进程进行负调节的作用。

Objective To study the function of the new gene p15rs on the proliferation of HeLa cells,which is related to CKIp15 INK4B. Methods The coding region of p15rs was cloned and transfected into HeLa cells.The growth curve,cell cycle and colony forming of the cell model were tested. Results The cell model HPS21 overexpressing p15rs was constructed.The overexpression of p15rs inhibited the growth rate of HeLa cell obviously.The double time of proliferation in HPS21 was delayed.Compared with control HPC4,HPS21 cells in G1 phase increased but which in S phase decreased.Furthermore,the capacity of growth in soft agar of HPS21 was strongly decreased.Conclusion The new gene p15rs may suppress the proliferation of tumor cell and negatively regulate the cell cycle.