低氧下小鼠肝细胞的细胞化学变化

CYTOCHEMICAL CHANGES OF HEPATOCYTES IN MICE EXPOSED TO HYPOXIA

目的 探讨低氧时小鼠肝细胞的细胞化学变化。 方法 实验用雄性小白鼠 45只 ,低氧组 36只 ,对照组 9只 ,低氧仓氧浓度为 10 %。用组织化学和图象分析方法 ,检测小鼠在低氧条件下肝细胞PAS ,乳酸脱氢酶(LDH) ,细胞色素氧化酶和镁离子依赖性ATPase活性。 结果 低氧组乳酸脱氢酶活性随低氧时间延长而升高 ;肝细胞色素氧化酶活性随低氧时间延长而降低。 结论 10 %低氧浓度可造成小白鼠肝细胞损伤 ,出现明显的细胞化学改变。

Objective To study the cytochemical changes of hepatocytes in mice exposed to hypoxia. Methods The mice were divided into two groups(n=45, all males, hypoxia group=36, control group=9). The mice of the hypoxia group lived in hypoxia (O 2:10%). Using imaging analytical instrument LEICA-500IW, We observed cytochemical changes of the hepatocytes. The PAS, LDH, cytochrome oxidase, Mg 2+ dependent ATPase activity of hepatocytes was assayed. Results Compared with the controls, the LDH activity of hepatocytes increased dramatically, the longer the hypoxic time, the higher the LDH activity of the hepatocytes; the cytochrome oxidase activity of hepatocytes decreased sharply, the longer the hypoxic time, the lower the cytochrome oxidase activity of the hepotocytes.Conclusion Hypoxia can injury the hepatocytes of the mice and result in sharp changes of the hepatic cytochemistry.;