人生长激素的真核细胞表达质粒的构建及鉴定

Construction and identification of eukaryotic expression plasmid of human growth hormone

目的 构建人生长激素的真核细胞表达质粒。方法 用双酶切从 pUC19-GHcDNA中克隆出生长激素基因 ,然后定向插入真核细胞表达载体pcDNA3中 ,行酶切和测序以鉴定。结果 重组的真核细胞表达质粒 pcDNA3-GHcDNA所含的GHcDNA序列和插入方向均正确。结论 本实验所构建的重组真核细胞表达质粒

Objective To construct eukaryotic expression plasmid for human growth hormone(hGH). Methods GHcDNA was cleaved by two kinds of restriction enzyme from pUC19-GHcDNA and inserted into eukaryotic expression vector pcDNA3,froming recombinant pcDNA3-GH plastid.Its sequence was identified by cleaving of enzyme and sequencing.Results It was demonstrated that GHcDNA sequence and its insertion direction in recombinant pcDNA3-GH plasmid were correct.Conclusion The recombinant pcDNA3-GH plasmid constructed in our test room possesses a certain significance for the study of its physical and pathological roles in vivo.