籼型谷秆两用稻201高频再生系统的初步研究

A preliminary study on establishing high regeneration frequency system of grain-straw-dual-use-rice 201, an indica rice

以籼型谷秆两用稻 2 0 1 (稻草粗蛋白含量比一般品种高 1倍左右 ,稻谷产量、品质与一般推广品种相当的新型水稻 )的幼胚作为试验材料 ,研究了基本培养基、琼脂质量浓度、激素、继代次数、培养基有机成分的处理方法等对其愈伤组织分化频率的影响 ,探索了谷秆两用稻高频再生体系 .此外还比较了 2 0 1与其它籼稻品种相同发育时期的幼胚在相同培养基上诱导愈伤组织的差异 .结果表明 ,NB是较合适的基本培养基 ,NB+0 .0 5 mg· L- 1 NAA +2 mg· L- 1 6-BA是较合适的分化培养基 ;适当提高分化培养基琼脂质量浓度 ,减少继代培养次数 。

Grain straw dual use rice (GSDUR) 201, an indica rice, has its straw in which the crude protein content is twice as high as that in the ordinary varieties, and its grain yield and quality which are the same as those of the ordinary popularized varieties. Its young embryos were used as test materials to research the effects of treatments with basal medium, agar mass concentration, hormone, subcultures and medium organic components on its callus differentiation frequency, and to detect the high regeneration frequency system of GSDUR. In addition, the difference of callus induction on the same medium during the same growth period of young embryos of 201 and other indica rice varieties was compared. The results indicated that NB was the most suitable basal medium and NB+0.05 mg·L -1 NAA+2 mg·L -1 6 BA was the most suitable differentiation medium for the immature embryos of GSDUR, and that properly increasing the agar mass concentration of the differentiation medium, decreasing the subcultures, and ultra infiltrating the organic components of the medium were helpful to callus differentiation and explants regeneration.