压电石英晶体DNA传感器的性能研究

Performance Research of A Piezoelectric Quartz Crystal DNA Biosensor

目的 为了了解利用DNA传感器进行临床疾病检验的可行性 ,对压电石英晶体DNA传感器的性能进行了研究。方法 利用自组装法 ,将 5′末端用巯基醇标记的单链DNA固定在镀金的压电石英晶体表面 ,再用该传感器来杂交检测溶液中的互补单链DNA和单碱基错配的互补单链DNA ,并用 0 1mMHCl对杂交后的传感器进行了再生。结果 4 4 3MHzAT切压电石英晶体DNA传感器检测灵敏度可达 80ng mL ,重复检测误差不超过±5Hz。在 5 0～ 4 0 0ng mL的浓度范围内 ,响应信号与检测浓度线性相关 ,并具有良好的特异性。杂交的DNA传感器能反复再生再利用 3～ 4次。

Objective This research was planed to study the possibility of disease diagnoses with piezoelectric quartz crystal DNA biosensor.Method A single strand DNA marked with mercaptan at the 5′ end was immobilized on the surfaces of gold plated piezoelectric quartz crystal by means of self assembly.The complementary single strand DNA and single base mismatch complementary single strand DNA in the solution were hybridized with this DNA biosensor.0 1mMHCl was used to regenerate the DNA biosensor after hybridization.Results A sensitivity of 80ng/mL can be achieved with a 4 43MHz AT cut piezoelectric quartz crystal DNA biosensor.At the same concentration,the deviation of repeated measurements is less than ±5Hz.Between 50～400ng/mL,there is linear relation between detected signals and DNA concentrations.The hybrid DNA biosensors can be regenerated and reused for 3～4 times.