地塞米松对PMA诱导CD18表达的抑制作用

Inhibitory effects of dexamethasone on the expression of integrin CD18 induced by PMA

目的 :研究GC抑制CD18表达的作用。方法 :选用PMA刺激下的U937细胞为实验模型 ,运用定量RT -PCR、Northern杂交检测U937细胞CD18mRNA的表达水平。结果 :Dex能明显抑制PMA诱导CD18mRNA的表达 ,这种抑制作用具有明显的剂量依赖性 ,GR的拮抗剂RU384 86能够明显扭转Dex的抑制作用。结论 :Dex通过GR介导能在转录水平上抑制PMA诱导的CD18mRNA的表达 ,该作用可能与糖皮质激素受体 (GR)在转录水平拮抗NF-κB有关。

AIM: To study the effect of dexamethasone(Dex) on the expression of CD18. METHODS: Quantitative RT-PCR analysis, Northern blotting technique were used to measure the expression of CD18 in U937 cells treated by PMA. RESULTS : Dex could significantly attenuated the effects of PMA in a dose-dependent manner (10 -6 mol/L-10 -10 mol/L). These effects of Dex (10 -7 mol/L) were completely aborted by RU-486 (10 -6 mol/L).CONCLUSION: Dex, via GR, could inhibit CD18 mRNA expression in U937 cells treated by PMA. The effects of Dex might be possibly depended on the counteracting action on the NF-κB.