巨细胞病毒对骨髓基质细胞粘附功能的影响

Effects of cytomegalovirus on the adhesion of bone marrow stromal cells

目的 :探讨巨细胞病毒 (CMV)对骨髓基质细胞表面粘附分子ICAM - 1和VCAM - 1的表达及骨髓基质细胞对正常造血细胞的粘附率的影响。方法 :用流式细胞仪检测骨髓基质细胞表达粘附分子ICAM - 1和VCAM- 1阳性率 ,用MTT方法检测骨髓基质细胞对正常骨髓造血细胞的粘附率。结果 :本实验所用的CMV可以感染骨髓基质成纤维细胞 ;10 0TCID50 剂量以下CMV对骨髓基质细胞无明显破坏作用 ;活性CMV感染骨髓基质细胞早期 (18h)粘附分子ICAM - 1表达明显较高 ,晚期 (12 0h)ICAM - 1表达明显较低 ;灭活的CMV也能使骨髓基质细胞ICAM - 1表达增高 ,作用与有活性的CMV相似 ;骨髓基质细胞在CMV感染早期 ,对造血细胞的粘附率增高。CMV对VCAM - 1的表达无明显影响。结论 :CMV感染骨髓基质细胞早期 ,骨髓基质细胞对造血细胞粘附能力增高 。

AIM: To investigate the effect of cytomegalovirus (CMV) on the expression of ICAM-1 and VCAM-1 in bone marrow stromal cells and on the adhesion of bone marrow stromal cells to hematopoietic cells. METHODS: Flow cytometry was used to detect the expression of adhesion molecules ICAM-1 and VCAM-1 in bone marrow stromal cells, MTT method was used to perform the adhesion assay of bone marrow stromal cells to normal hematopoietic cells. RESULTS: Bone marrow stromal cells could be infected by the CMV used in this experiment; CMV below the dose of 100TCID 50 could not destroy bone marrow stromal cells apparently; The expression of ICAM-1 increased at the early stage(18 h) of CMV infection, the expression of ICAM-1 decreased at the late stage (120 h) of CMV infection. Inactived CMV could also increase the expression of ICAM-1 as alive CMV; The adhesion rate of bone marrow stromal cells to hematopoietic cells increased at the early stage of CMV infection. CMV had no significant effects on the expression of VCAM-1 in bone marrow stromal cells. CONCLUSION: The adhesion capacity of bone marrow stromal cells to hematopoietic cells increased at the early stage of CMV infection, while the adhesion capacity of bone marrow stromal cells to hematopoietic cells decreased at the late stage of CMV infection.