严重挤压伤大鼠早期心脏损伤的某些发病机制的探讨

Pathogenesis of early cardiac myocyte damage after severe crush injury

目的 :观察挤压伤大鼠血浆心钠素 (ANP)、内皮素 - 1(ET - 1) ,血清心肌肌钙蛋白I(cTnI)浓度的改变 ,阐明心钠素、内皮素与心脏损伤的关系。方法 :复制挤压伤大鼠动物模型 ,全自动生化分析仪检测血清心肌酶改变 ,放射免疫法检测血浆ANP ,ET - 1水平 ,化学发光法检测血清cTnI浓度。结果 :挤压伤后 12h ,血浆ET - 1显著低于对照组 ,血浆ANP、血清心肌酶、cTnI水平显著高于对照组 ,并持续 4 8h以上。结论 :肢体挤压伤早期存在心肌细胞的损伤 ,其损伤可能与ANP、ET - 1有重要关系。

AIM: To study the changes of plasma atrial natriuretic peptide(ANP), endothelin-1 (ET-1) and serum cardiac troponin I(cTnI) levels and the effects of ANP and ET-1 on myocardium in crush injury rats. METHODS: Crush injury was produced in SD rats,the serum levels of cardiac enzyme and cardiac troponin I,plasma levels of ANP and ET-1 were studied by automated biochemical analyzer,automated chemiluminescence assay and radioimmunoassay. RESULTS: The plasma ET-1 level was much lower ,the levels of plasma ANP and serum cardiac enzyme and cTnI were much higher after crush injury than those of the control group( P< 0.05). CONCLUSION:ANP and ET-1 may play an important role in cardiomyocyte injury in the early stage of crush injury.