一氧化氮合酶抑制剂对大鼠局灶性脑缺血损伤的影响

Effects of nitric oxide synthase inhibitors on focal cerebral ischemic injury in rats

目的 :观察一氧化氮 (NO)和不同类型的一氧化氮合酶 (NOS)抑制剂对大鼠局灶性脑缺血损伤后的影响。方法 :用线栓法复制大鼠大脑中动脉脑缺血 (MCAO)模型后注射氨基胍 (AG)和NG-硝基 -L -精氨酸 (L -NA) ,观察大鼠脑缺血后脑梗死体积和脑组织中NO、MDA含量和NOS、SOD活性的变化。结果 :AG有明显的减轻局灶性脑缺血损伤作用 ;L -NA在缺血后 1h、6h时给药 ,有保护作用 ,但在 3h时给药 ,无显著改变。结论 :AG对局灶性脑缺血损伤有治疗作用 ;L -NA在不同时间给药 。

AIM:To observe the effects of nitric oxide and different isoforms of nitric oxide synthase inhibitors on the focal cerebral ischemic injury in rats. METHODS: After the rat model of focal cerebral ischemia were established with middle cerebral artery occlusion (MCAO), aminoguanidine(AG)and N G-nitro-L-arginine(L-NA )were administrated and the cerebral infarct size, NO production,MDA content, nitric oxide synthase(NOS) and SOD activities in the focal ischemic brain tissues were examined. RESULTS: AG could significantly attenuate the focal cerebral ischemic injury, and L-NA had a protective effect when it was administrated at 1 h,6 h but not at 3 h after surgery. CONCLUSION: Cerebral ischemic injury could be attenuated by both selective and nonselective inhibition of NOS.