蝉拟青霉对大鼠腹腔及肺泡巨噬细胞的激活作用

The activative effect of Paecilomyces cicadae on peritoneal and alveolar macrophages of rats

目的 :探讨蝉拟青霉菌丝体水提取物对大鼠腹腔、肺泡巨噬细胞的激活作用。方法 :大鼠随机分为4组 :正常对照组 (Ⅰ )、环磷酰胺组 (Ⅱ )、蝉拟青霉组 (Ⅲ )、环磷酰胺加蝉拟青霉组 (Ⅳ ) ,各组大鼠在同等条件下饲养 ,连续皮下注射相应药物 2 6d。用生理盐水灌洗大鼠的腹腔和肺泡 ,收集腹腔与肺泡巨噬细胞 (PMΦ、AMΦ) 37℃培养 2h ,用生化方法测定巨噬细胞内酸性磷酸酶 (ACP)和乳酸脱氢酶 (LDH)的活力 ,并进一步观察巨噬细胞摄取中性红的能力。结果 :蝉拟青霉菌丝体水提取物使正常大鼠PMΦ、AMΦ内ACP、LDH活力显著升高 ,并能拮抗由于使用环磷酰胺所致的降低作用 ;而且能显著增强大鼠PMΦ、AMΦ的吞噬能力。结论 :蝉拟青霉对腹腔。

AIM: To explore the activative effect of water extract from cultured mycelium of Paecilomyces cicadae (P. cicadae) on peritoneal macrophages (PMΦ) and alveolar macrophages (AMΦ) of rats.METHODS: The rats were randomly divided into four groups: normal control group(Ⅰ), cyclophosphamide (Cy) group (Ⅱ), P. cicadae group(Ⅲ), Cy+ P.cicadae group(Ⅳ).The rats were bred in the same circumstance and were administered with corresponding drugs by subcutaneous injection for 26 days. PMΦ and AMΦ of rats were irrigated by normal saline and collected by centrifuge and incubated in a humidified incubator for 2 h at 37 ℃. The activities of acid phosphatase (ACP) and lactate dehydrogenase (LDH) in the PMΦ and AMΦ were determined by biochemical methods, and the capability of PMΦ and AMΦ in phagocytosis of neutral red were measured by colorimetric method too. RESULTS: After administering P. cicadae to rats, the activities of ACP and LDH in PMΦ and AMΦ of normal rats were elevated significantly,and the reduction of the activities of ACP and LDH in PMΦ and AMΦ of rats due to Cy was notably antagonized, and the capability of PMΦ and AMΦ phagocytizing the neutral red were strengthened significantly.CONCLUSION: P. cicadae can activate the PMΦ and AMΦ of rats.