FK506抑制同种异体角膜缘移植术兔外周血T淋巴细胞CD25的表达

FK 506 inhibiting the expression of CD25 on peripheral blood in rabbitsof limbal allograft transplantation

目的 探讨FK506滴眼液对同种异体角膜缘移植术后的免疫抑制作用。方法 新西兰兔48只48眼建立角膜缘缺陷症动物模型。随机分为FK506组、环孢霉素A(CsA)组及生理盐水对照组,每组16眼。1个月后实施同种异体角膜缘移植术,检测术前及术后1－8周兔外周血T淋巴细胞上CD25的表达。结果 各组表达CD25的外周血T淋巴细胞数均有升高。对照组较其他两组高;而CsA组显著高于FK506组(P＜0. 05)。 结论 FK506滴眼液可抑制同种异体角膜缘移植术后T淋巴细胞上CD25的表达,其免疫抑制效果优于CsA。

Objective To discuss the effects of the immunosuppression of FK506 eye drops on limbal allograft transplantation. Methods Limbal deficiency models were established in 48 New Zealand rabbits (48 eyes) , which were randomized into 3 groups: FK506 group, CsA group and physiological saline control group, each group consisting of 16 eyes. Limbal allograft transplantations were performed one month later. The expression of CD25 on peripheral T cells was identified before and after operation 1 to 8 weeks. Results The expression of CD25 on peripheral T cells was increased in all 3 groups after operation. It was higher in control group than the other two groups,and that in CsA group was higher compared with that in FK506 group (P<0. 05). Conclusion Topically administed FK506 eye drops early after limbal allograft transplantation can lower the expression of CD25 on peripheral T cells. Its immunosuppressive effects are better than CsA.