摘要
建立了胃炎合剂中庆大霉素C组分的反相高效液相色谱测定法。利用离子交换树脂柱将庆大霉素和其它成分分离后,用2,4-二硝基氟苯(FDNB)柱前衍生化反相HPLC法测定庆大霉素C组分的含量。色谱条件:固定相为Irregular-H C_(18)柱;流动相为0.005 mol/L十二烷基磺酸钠溶液-高氯酸-甲醇(75:3:22,三乙胺调节pH 3.10);流速:1.0 m1/min;紫外检测波长:365 nm;柱温:20℃。结果:庆大霉素C组分的4个主要成分在20 min 内完全分离,平均方法回收率为99.05％～100.12％,日内、日间精密度RSD均小于5％(n=5)。结论:方法简便、快速、准确,可做为胃炎合剂的质量控制方法。
Objective: The purpose is to improve a RP-HPLC method for the determination of gentamicin C of gastritis mixture. Method: An ion- exchange resin method detaching gentamicin from distrurbances and RP- HPLC method has been established for the analysis of gentamicin C using FDNB pre-column derivatization. The column used Irregular-H Cu as the stationary phase. The mobile phase consisted of 0. 005 mol/L Sodium Iaurysulfonate solution-perchloric acid-methanol (75 : 3 = 22 pH adjusted to 3. 10 by triethylamine). The flow rate was 1. 0 ml/min and the wavelength of UV detection was 365 nm and column temperature was 20℃-Results:The main components of gentamicin C were completely separated from each other within 20 min. This method recovery rate was 99. 05-100. 12%. Boto intra-day and inter-day RSD of gentamicin C was less than 5%(n = 5). Conclusion: The method was simple, rapid and accurate. It could be successfully used for quantitative analysis of gastritis mixture.
出处
《江苏药学与临床研究》
2001年第4期17-19,共3页
Jiangsu Pharmacertical and Clinical Research
