先兆子痫患者外周血Th1Th2细胞亚群功能变化研究

Functional change of Th1,Th2 subpopulations in the peripheral blood of patients with preeclampsia.

目的 探讨先兆子痫患者Th1、Th2细胞亚群功能变化。方法 采用ELISA方法检测 1 7例先兆子痫患者及 1 5例正常孕妇PBMC培养上清液中IFN γ和IL 4的水平。结果 先兆子痫患者IFN γ水平升高 ,差异非常显著 (P <0 0 1 ) ;IL 4水平下降 ,差异非常显著 (P <0 0 0 1 ) ;IFN γ/IL 4比值升高 ,差异非常显著 (P <0 0 0 1 ) ;且IFN γ水平与平均动脉压呈显著正相关 ,r为 0 5 4 6 (P <0 0 1 ) ;IL 4水平与平均动脉压呈显著负相关 ,r为 - 0 5 4 4 (P <0 0 1 ) ;IFN γ/IL 4比值与平均动脉压呈显著正相关 ,r为 0 786 (P <0 0 1 )。结论 先兆子痫患者Th1、Th2细胞亚群功能失衡 。

Objective To study the functional change of Th1,Th2 subpopulations in patients with preeclampsia.Methods 17 patients with preeclampsia and 15 healthy pregnant women were selected randomly.IFN γ and IL 4 levels in cell culture supernatants of PBMC were detected by ELISA.Results Compared with those of normal pregnancy,IFN γ in preeclampsia increased significantly(P<0\^01);while IL 4 decreased significantly(P<0 001).In addition,IFN γ/IL 4 increased significantly(P<0\^001);IFN γ,IL 4 and IFN γ/IL 4 correlated significantly with MAP(r=0\^545,r=-0\^544,and r=0\^786,P<0\^01,P<0\^01,P<0\^01,respectively).Conclusion There is functional imbalance of Th1,Th2 subpopulations in patients with preeclampsia,and the extent of imbalance was correlated with severity of the disease.