具有羟基磷灰石结合活性的拟TGF-β多肽分子的实验研究

A pilot study of a synthesis peptide with TGF-β and hydroxyapatite binding activity

目的 :基于ECM蛋白和某些细胞因子结构功能的研究 ,设计合成了具有双功能多肽分子P18,通过此途径改善HA类骨修复材料及种植体HA涂层的生物活性。方法 :运用固相多肽合成技术合成了具有双功能基团多肽分子P18,对于其生物活性以及与HA结合的特性进行了初步研究。结果 :该分子和HA具有较高的亲和力 ,在生理条件下不能被洗脱 ,能够影响凝胶系统体外矿化作用 ,同时P18具有TGF β样活性 ,能够抑制水貂肺上皮细胞的增殖 ,低浓度促进UMR 10 6细胞株的增殖 ,但是高浓度作用减弱 ,该分子增加UMR 10 6细胞株碱性磷酸酶活性并能缩短ALP活性达到峰值的时间 ,并能够促进骨钙蛋白和Ⅰ型胶原的表达。结论 :所合成的多肽分子具有所设计的双功能 ,可以作为HA类骨损伤修复材料以及种植体涂层的生物活性添加成分。

Objective:To search after a kind of new method to improve the bioactivity of hydroxyapatite based biomaterials or coating layer of implants. Methods: A new peptide named P18 with two functional group was synthesized by employing solid phase peptide synthesis and its bioactivity was investigated. Results: Synthetic peptide has high binding ability with hydroxyapatite and cannot be eluted under physiological condition. The peptide can affect the in vitro calcification of gel system and also has TGF-β like activity. The TGF-beta activity was proved by the inhibition of the proliferation of Mv1Lu cell line. It can promote the proliferation of UMR-106 cell line, but the effect is lessened at higher concentration. Under the effect of the peptide, the activity of alkaline phosphatase was higher than control and the time required to reach peak value was shortened. Conclusion: The synthesized peptide possess two functions as design, it may have extensive application prospect as an ingredients of HA based materials or coating layer of implant.