白介素10对急性肺损伤保护作用的实验研究

The protective effects of interleukin-10 on acute lung injury induced by intratracheal instillation of LPS

目的 :探讨白介素 10 (IL 10 )对急性肺损伤 (ALI)的保护作用。方法 :气道内滴注内毒素 (LPS ,10mg/kg)建立大鼠ALI模型。 5 4只雄性SD大鼠随机分为对照组、内毒素组、内毒素加IL 10组 ,每组 18只 (2h、6h、2 4h各 6只 )。按时相处死并采集标本 ,观察大鼠体重、动脉血气分析、肺系数、支气管肺泡灌洗液 (BALF)总蛋白水平、BALF细胞总数及分类计数、血清及BALF中TNF α水平、肺病理。结果 :内毒素组大鼠体重、动脉血氧分压 (PaO2 )进行性降低 ,肺系数、BALF总蛋白水平、BALF细胞总数均明显增加 ,分类以中性粒细胞 (PMN)为主 ,血清及BALF中TNF α水平明显升高 ,肺病理示肺内PMN大量浸润伴出血、透明膜形成。IL 10组的各项指标均较内毒素组减轻。结论 :①气道内滴注内毒素可成功复制ALI模型。②BALF的TNF α水平比血中TNF α水平与肺损伤具有更好的相关性。③IL 10可减轻LPS诱导的ALI。

Objective:To investigate the protective effects of interleukin-10 on ALI. Methods: A rat model of ALI was induced by intratracheal instillation of LPS with a dose of 10mg/kg. 54 male S-D rats were randomly divided into control group?LPS group and LPS+IL-10 group,every group included 18 rats(6 rats at 2 hours?6hours and 24hours respectively).the body weight of rat?arterial gas analysis?the lung coefficient?the total protein concentration in BALF?the total cell counts and classification in BALF?the level of TNF-αin serum and BALF?lung pathology were examined. Results: In LPS group the body weight and PaO 2 were progressively decreased, the lung coefficient?the total protein concentration in BALF?the total cell count in BALF? the level of TNF-αin serum and BALF were greatly increased, lung pathology showed severe polymorphonuclear leukocytes infiltration with bleeding and hyaline membranes. Conclusion:①Intratracheal instillation of LPS may serve as a very relevant model to study ALI. ②TNF-α in BALF may has more significance than TNF-α in serum on assessment of lung injury. ③IL-10 could relieve ALI of rats.