乳糖酶水解乳清合成低聚半乳糖

ENTHYMIC SYNTHESIS OF GALACTOOLIGOSACCHARIDES(GOS)FROM WHEY

利用乳糖酶转移半乳糖苷的作用 ,以高浓度的乳清为原料 ,研究了温度、p H、乳糖浓度、酶浓度及反应时间对 GOS(低聚半乳糖 )合成的影响。通过实验发现 ,温度、p H、反应时间对 GOS合成影响不明显。酶浓度和乳糖初始浓度对产生有一定的影响。在优化条件下 ,GOS占总糖的 2 3.9%。

The experiments were designed to study effects of temperature,pH,initial lactose concentration,enzyme concentration and reaction time for the enzymic synthesis of GOS from high concentration whey.It was discovered that the temperature,pH and reaction time had no significant effects,however,enzyme concentration and initial lactose concentration had some effects for the prodection of GOS.At the optimized condition,GOS content in the end product is 23.9%.