摘要
本文报道了茶树组织培养中,采用种子子叶柄为材料,分化形成大量胚状体。胚性细胞团和植株再生的方法和结果。并筛选出了加速小苗生长和发根培养的较理想的培养基。
The paper reports the following: 1. The embryoids and embryonic calli form directly from cotyledon petiolein 1/2 MS medium with BA 1 ppm, IBA 2 ppm, GA_3 3 ppm, LH 40 ppm, andthen embryoids develop into plantlets in the same medium. A lot of adventitousbuds form on the surface of embryonic callus in N_6 medium with BA 10 ppm,NAA 1.2 ppm and LH 40 ppm. 2. A large number of calli are induced from cut of shoot in 1/2 N_6 mediumwith BA 4 ppm, IBA 2 ppm, GA_3 3 ppm and YE 200 ppm. A lot of embryoidsand embryonic calli are obtained by transplanting the callus into MS mediumwith BA l ppm, IBA 2 ppm, GA_3 3 ppm and YE 200 ppm, and then shootsform from embryonic calli in the same medium. A large number of buds form fromembryoid in 1/2 MS medium with BA 2.5 ppm, NAA l ppm and LH 40 ppm. 3. N_6 medium with BA 4 ppm, IBA 2 ppm is ideal for promoting plantletgrowth. 4. 1/2MS medium with Ad 5 ppm, NAA l ppm is optimum for root formation.
