FMMU白化豚鼠免疫学特性研究

Study on the Immunological Features of FMMU Albino Guinea-Pig

目的 通过测定补体含量、免疫球蛋白含量、淋巴细胞的转化率及红细胞免疫功能 ,比较FMMU白化豚鼠和花色豚鼠的免疫学特性。方法 利用自动生化分析仪测定FMMU白化豚鼠和普通花色豚鼠的免疫球蛋白含量 (IgG、IgA、IgM)、C3、C4含量及总补体水平 ;利用淋巴细胞转化实验测定淋巴细胞的转化率 ;利用红细胞免疫复合物 (immunocomplex ,IC)花环形成试验测定两种豚鼠红细胞免疫粘附功能。结果 FMMU白化豚鼠血清中免疫球蛋白含量 (IgG、IgA、IgM)、C3、C4含量及补体总活性均显著低于花色豚鼠 ;FMMU白化豚鼠淋巴细胞转化率比花色豚鼠略低 ;FMMU白化豚鼠红细胞C3bR花环形成率与花色豚鼠差异无显著性 (P >0 0 5 )。而RBC IC花环率显著低于花色豚鼠 (P <0 0 5 )。结论 封闭群FMMU白化豚鼠具有独特的免疫学特性 ,总体免疫学功能低于花色豚鼠。

Objective To compare the immunological features of FMMU albino guinea\|pig and pigment ones, including the serum content of complement and immunoglobulin, lymphocyte transformation rate and immunological function of erythrocyte.Methods\ The content of immunoglobulin(IgG,IgA,IgM), complement 3(C3), complement 4(C4) and the total activity of complement of two groups were measured by Clinical Chemistry Analyzer; the lymphocyte transformation rate of FMMU albino guinea\|pig and pigment ones were measured by lymphocyte transformation test; RBC\|C3bR rosette \|forming rates of two groups were measured by red cell C3b receptor rosette test. Results\ The serum content of immunoglobulin(IgG,IgA,IgM), complement 3(C3), complement 4(C4) and the total activity of complement of the FMMU albino guinea pigs were lower than those in pigment ones. The transformation rate of the FMMU albino guinea pigs was a little lower than that of pigment ones. There was no significant difference in the values of RBC\|C3bR rosette\|forming rate between FMMU albino guinea\|pigs and pigment ones, but RBC\|IC rosette\|forming rate was lower than that in pigment ones. Conclusion\ Closed colony FMMU albino guinea\|pigs have their unique immunological features, and their immunological function is weaker than that of pigment ones as a whole.