摘要
目的:采用小鼠至大鼠心脏移植模型,研究脾切除联用免疫抑制剂对异种移植物存活时间的影响。方法:动物随机分为组,分别采用脾切除、来氟米特()或二者联用以观察对异种移植心脏存活时间的影响。法检测血清。7LefELISAIgG用免疫组织化学法检测。IgG结果:单行脾切除使移植心脏存活(±)天4.401.14(P,脾切除联用使移植心脏存活<0.05)CsA(±)天与组比较,4.750.95(IIP。脾切除联用显著延长移植心脏存活时间达(±)天,且显著降低受体血清>0.05)Lef8.202.17水平和移植物中沉积IgGIgG(P。加用不能显著增加脾切除联用的效果。<0.05)CsALef结论:脾切除可显著延长小鼠至大鼠移 植心脏存活时间。脾切除和具有协同免疫抑制作用,可降低移植后受体血清水平及移植物中沉积,延长异种移植物LefIgGIgG的存活时间。
Objective: To study the role of splenectomy and immunosuppressive drugs in mouse-to-rat cardiac xenograft model. Methods : The animals were divided into 7 groups: group I (n =5), used as control; group II (n=5), splenectomy at day 0; group III ( n =6), treated with cyclosporine A(CsA) 20mg/ kg, every other day from day 0; group IV (n=6), received leflunomide (Lef) 10mg/ kg/d, from day 0; group V ( n=4), combined splenectomy with CsA; group VI ( n =5), added splenectomy and Lef; and group VII ( n=5), combined splenectomy with Lef and CsA. The sera IgG were determined by ELISA. IgG were detected by immunohistochemistry. Results: ±Splen-ectomy alone significantly prolonged the xenograft survival [(4.401.14) d, P±<0.05 vs group I]. Treatment with splenectomy and CsA significantly improved xenograft survival [(4.750.96)d, P<0.05 vs group I; P>0.05 vs group II]. Combination of splenectomy with Lef significantly prolonged the ±xenograft survival to (8.202.17) d (P< 0.05 vs group I, II, III, IV and V, respectively), and significantly decreased the serum IgG levels and the IgG deposition in the xenograft (P<0.05). Administration of splenectomy, ±CsA and Lef led to a highly significant prolongation of xenograft survival to 8.602.20d, howerer, it had no significant difference compared with group IV. Conclusion: Splenectomy can prolong the cardiac xenograft survival in mouse-to-rat models. Splenectomy has synergistic immunosuppressive effect with Lef to decrease the serum IgG levels and the IgG deposition in the xenograft and effectively prolongs the xenograft survival.
出处
《重庆医科大学学报》
CAS
CSCD
2002年第2期126-128,共3页
Journal of Chongqing Medical University
关键词
异种移植
心脏移植
移植物排斥
脾切除
来氟米特
Xenotransplantation
Heart transplantation
Graft rejection
Splenectomy
Leflunomide