生长抑素类似物诱导人胆管癌细胞株凋亡及对胞内游离钙的影响

Apoptosis of human cholangiocarcinoma cell line induced by somatostatin analogue and its effect on intracellular free calcium in vitro

目的 探讨生长抑素类似物SMS2 0 1 995 (SMS)是否通过诱导人胆管癌细胞凋亡而抑制肿瘤生长 ,以及与细胞内游离钙的关系。方法 采用人胆管癌细胞系SK ChA 1,应用MMT比色法 ,AnnexinV FITC技术及激光共聚焦显微镜技术等方法进行检测和观察。结果 在SMS作用下SK ChA 1细胞生长受抑制。SMS处理SK ChA 1细胞后 ,AnnexinV标记的凋亡细胞增多。细胞凋亡的同时细胞内游离钙浓度升高。结论 SMS可能通过诱导SK ChA 1细胞 [Ca2 + ]i依赖性细胞凋亡 ,抑制胆管癌生长。

Objective To investigate whether apoptosis induced by somatostatin analogue (SMS201-995, SMS) will inhibit the growth of human cholangiocarcinoma and the relationship between apoptosis and intracellular free calcium in vitro . Methods MMT assay, Annexin V-FITC and laser scanning confocal microscopy were performed to identify the inhibitory effect of SMS on cell proliferation and the induction of apoptosis in human cholangiocarcinoma SK-ChA-1 cells. Results SMS significantly reduced the growth of SK-ChA-1 cells and increased the number of cells with annexin V-labeling. Meanwhile the intracellular free calcium concentration was increased. Conclusion SMS can inhibit the proliferation activity by inducing the apoptosis mediated by increase of intracellular free calcium in SK-ChA-1 cell line.