高热惊厥对大鼠学习行为的影响及苯巴比妥的干预效果

The influence of hyperthermic seizures on learning behavior and the interventional efficacy of phenobarbital in rats

目的 :探讨高热惊厥对大鼠学习行为的影响及苯巴比妥的干预效果。方法 :采用热水浴诱导大鼠惊厥模型。实验大鼠随机分为 3组 :高热处理组 ,苯巴比妥钠处理组和正常对照组。高热处理组依据是否发生惊厥分为高热惊厥组和高热未惊厥组。苯巴比妥钠组随机分为单纯苯巴比妥钠组和苯巴比妥钠 高热组。采用水迷宫试验 ,记录各组大鼠寻找潜伏期及出错情况。结果 :高热处理组及苯巴比妥钠处理组寻找平台潜伏期均较对照组明显延长 ,高热惊厥组显著长于高热未惊厥组、苯巴比妥钠 高热组、单纯苯巴比妥钠组 ,而后 3组之间差异无显著性 ;高热处理组和苯巴比妥钠处理组寻找平台出错率明显增加 ,高热惊厥组大鼠出错率明显高于高热未惊厥组、苯巴比妥钠 高热组、单纯苯巴比妥钠组 ,高热未惊厥组出错率又显著高于后两者 ,后两组之间差异无显著性。结论 :高热、高热惊厥、苯巴比妥钠均影响大鼠学习记忆功能 ,高热惊厥影响最大。苯巴比妥钠可有效预防高热惊厥的发生 。

SUMMARY Objective: To study the effect of hyperthermic seizures on rat's learning behavior and to explore the interventional efficacy of phenobarbital. Methods: The rats were randomly divided into hyperthermia treated, phenobarbital treated, and control groups. The hyperthermia treated group was subdivided into hyperthermia and hyperthermic seizure groups, according to whether the rats developed seizures during hyperthermia treatment. Phenobarbital treated group was subdivided into phenobarbital and phenobarbital hyperthermia groups. By a water maze test, latencies to escape onto the platform and errors were recorded. Results: Latencies to escape onto the platform were significantly delayed in rats in hyperthermic seizure group than those in hyperthermia, phenobarbital hyperthermia, phenobarbital, and control groups. No significant differences were observed among the groups of hyperthermia, phenobarbital hyperthermia and phenobarbital. Many errors to escape onto the platform were recorded in rats in hyperthermic seizure group than those in hyperthermia, phenobarbital hyperthermia, phenobarbital, and control groups. The rats in hyperthermia group had more errors than those in phenobarbital hyperthermia and phenobarbital groups. No significant differences of errors were demonstrated in phenobarbital hyperthermia from that in phenobarbital groups. Conclusion: Hy perthermic seizure, hyperthermia only and phenobarbital all can disturb the learn ability in rats. Phenobarbital can effectively prevent hyperthermic seizures and alleviate the impact of hyperthermia on learning.