人卵巢上皮癌细胞原代培养及细胞系BUPH:OVSC-1的建立

Primary culture of human ovarian epithelial carcinoma cells and establishment of an ovarian carcinoma cell line BUPH:OVSC-1

目的 :为人卵巢上皮癌的研究提供更多体外研究模型 ,确立卵巢上皮癌原代培养的条件 ,建立细胞系。方法 :将 2 5例卵巢上皮癌手术切除的肿瘤组织或腹水标本进行体外培养 ,比较不同组织培养方法的效果。对所建卵巢癌细胞系的形态学、染色体核型、生长增殖及裸鼠种植情况进行观察。结果 :建立一套人卵巢上皮癌原代培养方法 ,使其体外培养传代数扩展至 10～ 15代。经原代培养得到一株人卵巢癌细胞系BUPH :OVSC 1,其形态学、染色体核型分析、接种致瘤性的观察结果表明 ,该细胞系符合人体恶性细胞特征。裸鼠种植瘤病理切片该细胞呈肉瘤样细胞形态 ,电镜及角蛋白免疫组化证实其为上皮起源。结论 :应用改良的卵巢上皮癌原代培养方法能改善细胞的体外生存期。BUPH :OVSC 1是一株特殊的人卵巢癌细胞系 ,符合人卵巢肉瘤样癌细胞系的特征 。

SUMMARY Objective:To improve the primary culture and establish cell lines of human ovarian epithelial carcinoma cells, providing more experimental models for the investigation of human ovarian epithelial carcinoma.Methods:25 samples from patients with ovarian epithelial carcinoma were cultured in vitro and different tissue culture techniques were compared. The morphology,karyotype,proliferation kinetics and tumorigenecity in nude mice were observed. Results:The primary culture conditions of human ovarian epithelial carcinoma cells were defined that permitted a serial propagation. There were significant improvements of lifespan in vitro , providing a source of relatively abundant and pure ovarian carcinoma cells for further studies. Besides, a continuous cell line named as BUPH:OVSC 1 was obtained from primary culture. The morphological observation,karyotype analysis and tumor formation in nude mice demonstrated its malignant characteristics. The transplanted tumor was sarcomatoid by histological examination after HE staining. However, transmission electron microscopic observation and immunocytochemical stainings of cytokeratin revealed that the cells were of epithelial origin. Conclusion: In vitro lifespan of human ovarian epithelial carcinoma cells can be prolonged by improved primary culture method. BUPH:OVSC 1 was demonstrated as a new human ovarian sarcomatoid carcinoma cell line and could be a useful model for the study in human ovarian sarcomatoid carcinoma.