釉基质蛋白对人牙周膜细胞粘附、伸展的影响

Effects of enamel matrix proteins on the attachment and spreading of human periodontal ligament cells in vitro

目的 :了解釉基质蛋白对人牙周膜细胞黏附、伸展的影响。方法 :改良组织块法培养人牙周膜细胞 ,固相结合分析方法观察釉基质蛋白对人牙周膜细胞黏附、伸展的影响。结果 :实验组PDLC黏附率与对照组无差别 ,实验组PDLC伸展率高于对照组。结论 :EMPs对PDLC黏附无影响 ,但可促进其伸展。

AIM: To evaluate the effects of enamel matrix proteins (EMPs) on the attachment and spreading rate of human periodontal ligament cells(PDLC). METHODS: Cell culture technique and solid-phase binding assay were used to observe the ability of adhesion and spreading of the PDLC. RESULTS: In test groups there was no significant change in cell attachment ; but the cell spreading rate increased significantly. CONCLUSION: EMPs could enhance the spreading ability of the PDLC, but no effects on its attachment.