用高密度cDNA微阵列技术检测鼻咽癌差异表达基因

Gene Expression Profiling in Nasopharyngeal Carcinoma Determined by High Density cDNA Microarray

背景与目的:鼻咽癌的发生与发展涉及多个步骤、多个因素的参与,对此目前尚缺乏了解。本研究试图通过大规模检测鼻咽癌的基因差异表达来揭示鼻咽癌的差异表达基因谱,以发现与鼻咽癌发生、发展相关的新的候选基因。方法:用由18000个基因构建的高密度cDNA微阵列对21例鼻咽癌分3个样本池分别检测并与鼻咽非癌组织作对比分析,随机选2个基因用半定量RT-PCR验证检测结果。结果:3个样本池鼻咽癌差异表达基因分别为186个、95个和36个,总数达317个,表达上调的277个,表达下调的40个;差异表达基因中2个样本池以上有共同差异表达的基因有23个,表达上调的16个,表达下调的7个;其中3个样本池表达均有差异者6个,5个上调、1个下调,5个上调的基因中2个是已知基因,3个是未知基因,1个下调的是未知基因。结论:本研究揭示了鼻咽癌的基因表达谱,为了解鼻咽癌发生、发展的分子机理提供了大量信息,为寻找鼻咽癌相关基因提供了重要线索。

Background &Objective:Multiple steps and gene expression changes were involved in the development and progression of nasopharyngeal carcinoma (NPC), but the molecular mechanism is not understanded clearly.This study was designed to investigate the profiling of differently expressed genes in nasopharyngeal carcinoma compared with noncancerous nasopharynx tissues to expore the feature of gene expression in NPC and identify new candidat genes related to the development and progression of NPC. Methods: Differently expressed genes of NPC in 21 cases, which were divided into three pools, were investigated by using high density cDNA microarray representing 18 000 cDNA clones. The alterations in gene expression levels were comfirmed by reverse transcription PCR in 2 randomly selected genes. Results: A total of 317 genes were identified to be differently expressed in NPC of three pools as compared with noncancerous controls. Among the 317 genes, 23 genes were found to be consistent in at least two NPC sample pools, including 16 up regulated genes and 7 down regulated genes. Six genes were found to be consistent in all the three NPC sample pools. The 6 genes include 2 up regulated known genes,3 up regulated unknown genes and 1 down regulated unknown gene. Conclusion:The present study represents a global view of gene expression of NPC and provides important clues for further study of NPC related genes.