人乳腺癌及腋窝淋巴结组织GST-π蛋白表达的免疫组化研究

Immunohistochemistry Study of GST-π in Breast Cancer and Axillary Lymph Nodes

目的 :研究胎盘型谷胱甘肽S -转移酶 (GST -π)基因在人乳腺癌及其腋窝清扫的淋巴结中的蛋白表达与乳腺癌发生、发展、预后转归及临床耐药的相关性 .方法 :采用SP免疫组化方法检测乳癌组织及腋窝淋巴结组织GST -π蛋白表达 .结果 :在正常乳腺组织、乳腺良性肿瘤、乳癌组织、HE转移淋巴结、HE无转移淋巴结中GST -π蛋白阳性表达率分别为 :0 ,86 % ,5 9% ,77% ,43% .结论 :在乳癌启动和促进阶段GST -π表达增高 ,是一典型癌前病变标志酶 ;淋巴结转移癌细胞耐药性增强 ,HE阴性淋巴结中出现GST-π阳性表达提示预后不好 ,其阳性表达反映肿瘤发生的进程及耐药倾向 。

To study GST-π gene for micrometastases and drug resistance in breast cancer and lymph nodes. Methods: Samples were assayed for GST-π protein expression by immunohistochemistry. Results: Percentage of GST-π gene protein expression in normal breast tissue, breast benign tumor, breast cancer, HE positive and HE negative lymph nodes were 0.86%, 56%,77%, 43% respectively. Conclusion: Expression of GST-π protein shows high level in the early stage of breast cancer.