视网膜脱离病人视网膜下液及血清IL-6含量与T细胞亚群的关系

The relationship among the levels of IL-6 in subretinal fluid,serum and T cell subspecies

目的 探讨白介素 6 (IL- 6)及 T细胞亚群在视网膜脱离疾病中的免疫病理机制。方法 采用 MTT法和抗体致敏的红细胞花环试验 (间接法 )检测 52例视网膜脱离病人视网膜下液及血清中 IL- 6活性和外周血 T细胞亚群。结果 视网膜脱离患者的视网膜下液、血清中的 IL-6活性均高于对照组 (P<0 .0 1 ) ,且与病程成正比。外周血 T细胞亚群 CD4 + 细胞、 CD4 + / CD8+ 均明显降低 ,与血清及网膜下液中 IL- 6活性呈负相关 ,CD8+ 明显增高。结论 血清及网膜下液中 IL- 6活性与机体免疫功能密切相关。两者互为因果 ,共同参与视网膜脱离炎症病变的免疫病理损害。可作为判断疾病的预后和免疫调节治疗的客观指标。

Objective To investigate the immunopathology mechanism of white cell IL 6 and T cell subspecies in retinal detachment disease. Methods MTT technique and red cell leis experiment that were allergized by antibody (indirect method) were applied to detect the activity of IL 6 in subretinal fluid,serum and T cell subspecies in external blood of 52 patients with retinal detachment. Results The activity of IL 6 in subretinal fluid and serum of patients with retinal detachment is all higher than contrast groups ( P <0.01), and is positive relevant to the disease course. The levels of T cell subspecies in external blood, CD + 4 cells and CD + 4/CD + 8 all obviously reduced ,and were negative correlation with the activity of IL 6 in serum and subretinal fluid .The levels of CD + 8 cells obviously rose .Conclusions The activity of IL 6 in serum and subretinal fluid is closely correlative with the immune function of human body .They were causation mutually .Together they play parts in immunopathology injury of inflammatory changes of retinal detachment .They can become objective index that estimate disease prognosis and immune adjustment treatment.