EGb761对NO供体SNP诱导的海马神经元凋亡的保护作用

Protective effect of extract of ginkgo biloba on apoptosis of cultured hippocampal neurons induced by nitric oxide-donor SNP

目的 探讨银杏叶提取物 (EGb761 )对 NO供体硝普钠 (SNP)引起的大鼠海马神经元凋亡的影响。方法 采用 MTT比色分析测细胞存活率、 Hoechst 332 58荧光染色及 DNA琼脂糖凝胶电泳分析等方法检测凋亡。结果 不同剂量 EGb 761预处理海马神经元 6 h可剂量依赖地对抗 SNP引起的神经元凋亡 ,提高神经元的存活率 ;减少 SNP引起的核固缩、凝聚和碎裂现象 ;DNA凝胶电泳图谱未见典型的“梯子状”改变。结论 EGb 761对 NO供体

Objective To study the effect of extract of Ginkgo biloba (EGb761) on apoptosis of cultured hippocampal neurons induced by nitric oxide-donor, sodium nitroprusside (SNO). Methods Neuronal viability and morphology were tested by MTT assay and light microscope. Fluorescent staining with Hoechst 33258 and agarose gel electrophoresis was used to analyze apoptosis. Results Pretreatment with different concentrations of EGb761 (7.5-90ug/ml) for 6h increased the survival rate of neurons in a concentration-dependent fashion. EGb761 prevented the neuronal nuclei from shrinkage, condensation and cleavage induced by SNP. It also blocked neuronal nuclear DNA fragmentation induced by SNP. Conclusion EGb761 can protect neurons from apoptosis induced by NO.