雷公藤多甙对噁唑酮诱导小鼠肠炎模型脾淋巴细胞因子的作用及其机制

Multi-glycosidorum triptery suppresses production of interleukin-4 by splenocytes in oxazolone-induced murine colitis

目的 观察小鼠肠炎模型体外实验中 ,雷公藤多甙 (MGT)对唑酮 (oxazolone,OXZ)诱导的小鼠肠炎模型中脾脏淋巴细胞细胞因子分泌类型的影响 ,从免疫学、分子生物学角度探讨MGT治疗炎症性肠病 (IBD)的作用机制。方法 采用SJL/J小鼠 ,经直肠注入OXZ制成IBD模型 ;3d后将小鼠处死 ,即刻取出脾脏 ,收集脾细胞 ,将MGT(0 .1和 0 .0 1mg/ml两个浓度 )分别加入培养的淋巴细胞液中 ,行ELISA检测白介素 4 (IL 4 )和γ 干扰素 (IFN γ)。结果 1.MGT对IFN γ生成的影响 :(1)正常对照组 :未加入MGT组 (空白对照 )的IFN γ为 (1.2 4± 0 .13) pg/ml;0 .0 1mgMGT组为(0 .97± 0 .2 6 )pg/ml;0 .1mgMGT组为 (0 .87± 0 .18) pg/ml;(P <0 .0 2 ) ;(2 )OXZ肠炎模型组 :与正常对照组比较 ,未加入MGT组 (空白对照 )IFN γ显著降低 [(0 .6 5± 0 .0 8) pg/ml比 (1.2 4±0 .13) pg/ml,P <0 .0 1],0 .0 1mgMGT组和 0 .1mgMGT组IFN γ均低于空白对照 [分别为 (0 .4 7± 0 .0 5 ) pg/ml;(0 .4 6± 0 .0 9) pg/ml],但差异无显著性。 2 .MGT对IL 4生成的影响 :(1)正常对照组 :未加入MGT组 (空白对照 )的IL 4为 (5 .6 5± 0 .4 8) pg/ml;MGT有显著抑制作用 [0 .0 1mg/mlMGT组为(4.97± 0 .38) pg/ml;0 .1mgMGT组为 (3.98± 0 .

Objective To investigate the in vitro effect of multi glycosidorum triptery (MGT) on cytokine production by splenocytes of oxazolone (OXZ) induced colitis in murine model. Methods Six mg of OXZ (in 50% of ethanol) was administered in male SJL/J mice intrarectally to induce colitis and mice were sacrificed 3 days later. Isolated splenocytes were cultured for 24 hours in the presence of PMA and ionomycin, MGT of 0.1 mg/ml or 0.01 mg/ml was added to the culture medium of splenocytes. Production of IFN γ and interleukin 4 (IL 4) in the supernatant was measured by ELISA. Results The production of IFN γ was suppressed by both 0.01 mg/ml and 0.1 mg/ml of MGT [normal control ( 1.24 ± 0.13 ) pg/ml→(0.97±0.26) pg/ml→(0.87±0.18) pg/ml, P <0.02] in a dose dependent pattern. In OXZ induced colitis group, the basic level of IFN γ was significantly lower than that of normal control group [( 1.24 ± 0.13 ) pg/ml vs (0.65 ± 0.08) pg/ml , P < 0.01 ]; but IL 4 production was significantly increased [( 7.83 ±0.69) pg/ml vs ( 5.65 ± 0.48 ) pg/ml, P <0.01]. In both groups, MGT suppressed IL 4 production in a dose dependent manner [normal control group: (5.65±0.48) pg/ml→(4.97±0.38) pg/ml→( 3.98 ±0.32) pg/ml, P <0.01; OXZ group: (7.83±0.69) pg/ml→(7.07±0.47) pg/ml→(6.35± 0.48 ) pg/ml, P <0.01].Conclusions OXZ induced IL 4 overproduction by splenocytes was significantly suppressed by MGT in a dose dependent manner and the effect of MGT on ulcerative colitis might be related with the suppression of Th2 type (IL 4) mediated immunological response of splenocytes.