增殖细胞核抗原、CDK4、P16在毫米波照射肝肿瘤大鼠中的表达

Expression of proliferating cell nuclear antigen, CDK4 and P16 in rat hepatocellular carcinoma by milli-meter wave radiation

目的 观察毫米波照射对实验性肝肿瘤大鼠细胞增殖的影响。方法 4 0只Wistar雄性大鼠分为 4组。第 1至 3组为诱导肝肿瘤组 ,二乙基亚硝胺 (DEN)喂养 14周 ,第 1组为肿瘤对照组 ;第2、3组分别用毫米波照射大鼠肝区 10周和 5周 ;第 4组为正常大鼠毫米波照射组。检测肝肿瘤演变过程中肝匀浆的酶学改变 ,光学显微镜观察肝组织学的变化。免疫组化检测肝组织中增殖细胞核抗原(PCNA)、CDK4和P16。结果 肿瘤对照组有肝结节形成 ,诱导肝肿瘤毫米波照射组大鼠组织匀浆中γ GT 10周组为 13.75± 2 .4 1,低于肿瘤对照组的 18.4 4± 4 .88(P <0 .0 5 ) ;而 5周组为 16 .4 3± 2 .12 ,与肿瘤对照组无显著差异 (P >0 .0 5 ) ,诱导肝肿瘤照射组仅见嗜酸性和嗜碱性结节。毫米波照射后肝肿瘤大鼠PCNA、CDK4明显低于肿瘤对照组 ,P16蛋白表达较肿瘤对照组增多。结论 毫米波照射可调节抑制细胞的增殖 ,对诱导大鼠肝肿瘤有延缓作用。

Objective To study the expression of proliferating cell nuclear antigen (PCNA), CDK4 and P16 on rat hepatocellular carcinoma by millimeter wave radiation. Methods Fourty male Wistar rats were randomly divided into four groups. Group one to three were feeded by diethylnitrosamine (DEN). Group one was a tumor control group. In group two and three the liver was directly radiated by 35.8 GHz, 100 mW/cm 2 millimeter wave for 20 min, twice a week for 10 or 5 weeks. Group four was a normal control radiated group. Fourteen weeks later all rats were sacrificed to undergo serological test and immunohistochemical stain of liver. Results The serum levels of γ glutamyltransferase in group two and three were lower than that in group one. Adenocarcinoma was only existed in group one by histological examination of liver tissue. Other groups of DEN exposure only had basophilic and eosinophilic nodules. Liver tissue expression of PCNA and CDK4 in group two and three were significantly lower than in group one, but the expression of P16 in group two and three was higher than that in group one. Conclusions Radiation with millimeter wave can partially inhibit cell proliferation and suppress the DEN induced hepatocellular carcinoma in rats.