RANTES在狼疮肾炎肾组织中的表达及其意义

Expression of chemokine RANTES in renal tissue of lupus nephritis

目的 了解狼疮肾炎 (LN)肾组织中化学趋化因子激活正常T细胞表达和分泌因子(RANTES)的表达并探讨其与LN肾脏病理改变和肾功能损害的关系。方法 采用微波免疫组织化学染色 (SP法 )和原位分子杂交技术检测LN肾脏RANTES的表达 ,并进一步分析其与LN活动指数、肾脏病理和功能损害的关系。结果 狼疮肾炎组织中RANTES表达显著增高 ,以WHOⅣ型为显著。RANTES在肾小球和肾小管均有表达 ,但肾小管表达更显著。LN肾组织RANTES表达与肾组织活动指数、肾脏病理改变和肾功能损害显著相关。结论 RANTES可能参与了LN的发病机制 ,肾组织中RANTES的表达可作为反映狼疮肾组织活动病变和进行性肾损害的参考指标。

Objective To investigate the role of chemokine 'regulated upon activtion normal T cell expressed and secreted'(RANTES) in the pathogenesis of lupus nephritis (LN).Methods A microwave based immunohistochemistry (SP) and in situ hybridization were used to detect the expression of RANTES and renal in jures in LN and normal control.At the same time,the correlation berween expression of RANTES and renal injures in LN was examined.Results The expression of RANTES in LN was higher than that of normal control,the highest one was type Ⅳ LN.The number of positive cells expressing RANTES in renal tissue of LN showed a strong correlation with kidney activity of LN,histological changes and function lesion in LN,respectively.Conclusion RANTES may play an important role in the pathogenesis of LN,and the number of positive cells expressing RANTES may be considered as a predictor for the kidney activity and progressive renal injury in LN.