HBV转录后调节序列中与干扰素α应答有关位点的初步鉴定与分析

Preliminary identification and analysis of different points related with response to interferon-αin HBV post-transcriptional regulatory element

目的筛选乙型肝炎病毒(HBV)感染者转录后调节序列中与干扰素α治疗应答有关的差异位点。方法采用PCR反应产物直接测序法,对31例HBV感染者血清中HBV DNA上的转录后调节序列(HPRE)片段扩增后测序,与GenBank中来源于欧美人群的乙型肝炎序列进行比较,并结合临床资料进行分析。结果在中国慢性HBV感染者HPRE的β2区,存在3个位点变化,分别为nt1 504 (T→C),占75.0%;nt1 508 (C→T),占57.1%,C→G,占10.7%;nt1509 (C→T),占60.7%。而在欧美HBV感染者的HBV DNA序列中均不存在上述位点变化。HPRE位点差异的发生率与HBV DNA水平未见明显相关。结论不同种族HBV感染者转录后调节序列的差异可能与干扰素α治疗的应答性有关。

Objective To screen the differential points related with response to interferon-αin HBV post-transcriptional regulatory element (HPRE). Methods HPRE sequence of 31 Chinese patients with HBV infection were detected by direct sequencing of PCR products. Differential points in HPRE between Chinese patients and White patients were compared. Results The T to C differential point at nt 1 504 and C to T(G) at nt 1 508 were found respectively in HPRE of 21 and 19 patients with chronic hepatitis B, the C to T differential point at nt 1 509 were found in 17 cases. These differential points were not found in HPRE of the White patients. The relationship between the HPRE differential points and the levels of HBV DNA was not observed. Conclusion There were differential points in the HPRE between Chinese and White HBV patients, which might be correlated with response to interferon-α.