应用RD-PCR方法制备K562细胞表达谱芯片基因探针

Application of restriction display PCR in preparing the probes of the gene chip for inves- tigating gene expression profile of K562cells

目的收集K562细胞表达谱芯片的基因片段。方法培养K562细胞,抽提其mRNA,反转录为cDNA,应用限制性显示PCR技术(RD-PCR)分离K562细胞不同组别的产物片段,分别行PCR扩增。结果制备了可用于基因芯片制作的基因探针片段。结论RD-PCR方法适合于基因芯片探针的收集。

Objective To collect the gene fragments of the gene chips for investigating the expression profile of K562 cells. Methods The mRNA was extracted from cultured K562 cells to obtain cDNA by reverse transcription-PCR. The gene fragment products of different groups were acquired and isolated by reverse display PCR (RD-PCR). Result The probes for the gene chip were successfully prepared. Conclusion RD-PCR adapted in this study suits the needs for collecting the probes for the gene chips.