70%肝切除对大鼠胎肝细胞脾内移植后增殖能力的影响

EFFECTS OF 70% HEPATECTOMY ON THE PROLIFERATION OF RAT FETAL HEPATOCYTES AFTER INTRASPLENICAL TRANSPLANTATION

研究 70 %肝切除对大鼠胎肝细胞脾内移植后增殖影响。分离孕 3周SD大鼠胚胎肝细胞 ,将其移植入 70 %肝切除大鼠脾内 ,分别于移植后 7天和 30天应用流式细胞仪分析肝切除大鼠残肝细胞的细胞周期 ,用图像分析法检测脾内移植胎肝细胞面积密度。与对照组比较 ,胎肝细胞移植后 7天 ,肝切除鼠残肝细胞S期细胞比例明显增加 (P <0 0 5 ) ,Gz/M期细胞比例明显减少 (P <0 0 1)。而其脾内移植胎肝细胞面积密度则显著升高 (P <0 0 5 ) ;30天后 ,残肝细胞再生状态与移植胎肝细胞的面积密度与对照组比较均无显著性差异。研究表明 ,70 %肝切除的肝再生有利于大鼠胎肝细胞脾内移植后的增殖。

To study the effects of 70%Hepatectomy on the proliferation of rat fetal hepatocytes after intrasplenical transplantation, fetal hepatocytes isolated from 3 week SD rat fetuses bred were transplanted into the spleens of liver regeneration model rats with 70% partial hepatectomy. The cell cycle of hepatocytes in the remanent liver was analyzed by flow cytometer and the density dimensions of the donor fetal hepatocytes in spleen were measured by Image Analysis System 7 and 30 days post transplantation respectively. Compared with the control group, the proportions of S state cells in the remanent liver were obviously increased ( P <0 05), the proportions of G 2/M state cells were obviously decreased( P <0 01), but the density dimensions of the donor fetal hepatocytes in spleen were significantly increased( P <0 05) in rats with hepatectomy7 days post transplantation. However,Compared with the control group, the proportions of S and G 2 /M state cells in the remanent liver and the density dimensions of the donor fetal hepatocytes in spleen did not change significantly. The environment of liver regeneration after 70%Hepatectomyis propitiousto the proliferationof fetal hepatocytes after they are transplanted into spleen.