脑细胞缺血离体模型——缺血突触体模型的建立与评价

Establishment and Evaluation of a Model of Cerebral Ischemia in vitro-Ischemic Synaptosome Model

目的 :建立一种脑细胞缺血离体模型。方法 :大鼠脑突触体分别在不同条件下培养 ,电镜观察形态结构 ,测定其生物活性。结果 :突触体结构正常 ,内含线粒体与递质囊泡 ,缺血培养对形态结构无明显影响。突触体悬液与上清液LDH活性的比值在正常和缺血培养条件没有明显差异 ,而缺血培养可使突触体内游离钙浓度显著升高 (P <0 0 5) ,高钾刺激可使其进一步增高 (P <0 0 1 )。结论 :大鼠脑突触体经体外缺血培养后 。

Aim:To establish a model of ischemia cerebral cell in vitro. Methods: Synaptosomes prepared from rat brain were incubated in different conditions, respectively. The morphology of synaptosome was observed by electron microscope, and the biological activity was detected.Results:Intact synaptosomes were observed by electron microscope, and there were mitochondria and many of vesicles in it. Incubation without oxygen and glucose had no effect on its morphology. The ratio of LDH in suspension to in supernatant had no difference between those in normal incubation and ischemic incubation, but ischemic incubation increased [Ca 2+ ] i significantly ( P <0 05), and high concentrations of potassium can increase it remarkably ( P <0 01).Conclusion:Synaptosomes prepared from normal rat brain kept morphological intactness and biological activity after incubated without oxygen and glucose. Ischemic synaptosome model could be established successfully.