^(99m)Tc-MDP放射性骨显像观察重组人骨形成蛋白-2与珊瑚人工骨修复牙槽骨缺损

Evaluation of repairing alveolar bone defects with coral artificial bone composed of human recombinant bone morphogenetic protein-2 using dynamic ~ (99m ) Tc-MDP bone imaging

目的 探讨应用99mTc -MDP放射性骨显像观察重组人骨形成蛋白 - 2与珊瑚人工骨在牙槽骨缺损修复中成活和成骨效果的价值。方法 拔除 1 2只成年狗两侧上颌第 2及第 3切牙 ,并去除牙槽窝之间的牙槽间隔 ,一侧随即植入复合骨 ,对侧植入珊瑚人工骨 (珊瑚骨 )作为对照。并于植入后 4 ,8,1 2周取材 ,采用组织学观察、图像分析和99mTc -MDP核素骨显像等方法比较两种植入材料在牙槽窝中的骨修复能力。结果 复合骨植入牙槽骨后 ,材料被逐渐降解吸收 ,新骨不断生成 ,1 2周后 ,植入材料完全被成熟的骨组织取代 ;图像分析结果显示不同时间复合骨组新骨形成的比值显著高于珊瑚骨组 (P <0 0 5) ;4和 8周复合骨组核素浓聚程度高于珊瑚骨组 ,1 2周两组核素浓聚程度差异不明显。结论 99mTc -MDP放射性骨显像能早期判断移植骨的成活情况及成骨效果。

Objective To study the value of ~ 99m Tc-MDP bone scanning in monitoring the survival and bone repairing ability of coral artificial bone compoed of rhBMP-2(rhBMP-2/CAB) implanted into extraction sockets. Methods 12 adult dogs were used as experimental animals. Immediately after extraction of the upper second and third incisors, rhBMP-2/CAB and coral artificial bone(CAB) were respectively implanted into extraction socket on each side. The bone repairing ability of the two grafts was analyzed with histology, image analysis system and radionuclide bone imagining at 4th, 8th and 12th week respectively. Results rhBMP-2/CAB were absorbed gradually after they were implanted into alveolar bone defect and were replaced completely by bone at 12 weeks. The ratio of new bone formation of rhBMP-2/CAB was significantly higher than that of CAB at different timepoint(P<005). The uptake of ~ 99m Tc-MDP in rhBMP-2/CAB was higher than that of CAB at 4th and 8th weeks, but there were no significant differences between the two groups at 12th weeks. Conclusion The repairing ability and effect of rhBMP-2/CAB in extraction sockets are obviously better than those of CAB and radionuclide bone imaging is a major method for early assessment of bone graft survival.