聚乳酸载体在组织工程骺软骨研究中的应用

The application of pelylactic acid in the studies on the engineered growth plate

目的了解聚乳酸是否可以用作骨骺软骨细胞的载体。方法采用多孔聚乳酸膜片作载体,与骨骺软骨细胞共培养,通过组织学、透射电镜、扫描电镜观察细胞与载体的关系及细胞生长情况;通过凝胶渗透色谱法了解聚乳酸载体肌肉植入后分子量改变情况和局部反应。结果倒置显微镜、组织学及电镜观察均显示细胞可以在载体上生长,并且分泌基质;聚乳酸载体植入体内后分子量逐渐减少,周围异物细胞较少。结论首次成功地培养出了组织工程骺软骨;聚乳酸在体内可以不断降解,组织相容性较好;骨骺软骨细胞可以在载体上不断生长。

Objective To investigate the possibility that polylactic acid(PLA)serve as the scaffold to grow into engineered growth plate(EGP).Methods The porous PLA discs were seeded with growth plate chondrocytes and cocul tured for 3weeks.Then they were exam ined by microscopy ,SEM and TEM to determined the chondrocyte growth characteristics on the PLA scaffold.The change s of the molecular weight of the PLA scaffold after being implant ed into the muscles of rabbits was evaluated by GPC.The local tissue reaction to the polymer was observed as well.Results It was demonstated that the growth pl ate chondrocytes could keep prolife rating and secreting the extracellu ar matrix on the carrier.The molecular weight of the polymer was decreased gradually and the local tissue reaction was slight .Conclusions It was the first time that the EGP was obtained successful ly.The PLA was degradable in vivo and it' s biocompatibility is acceptable.T he growth plate chondrocytes could keep proliferating on the PLA s caffold.