成熟大鼠脑损伤后神经干细胞的表达与增殖

Expression and proliferation of neural stem cell induced following brai ninjury of mature rat

目的探讨成熟大鼠在脑组织受损后神经干细胞的表达与增殖情况。方法通过立体定向仪横断成熟大鼠皮质和内囊处的尾状核,于术后1、2、3、4、5周分别杀死动物。用Nestin免疫组化染色和Nissl染色的方法,观察损伤后成熟大鼠脑内神经干细胞的反映模式。结果成熟大鼠在损伤后伤侧侧脑室、对侧侧脑室和三脑室室管膜下区的神经干细胞明显分裂增殖,伤后1周最显著;在伤口处表达为一个以伤口为中心的梯度递减模式,伤后两周最显著。结论成熟大鼠脑损伤后可诱导脑室室管膜下区的神经干细胞增殖,进而表达为伤口周围的神经干细胞梯度递减反应模式,提示神经干细胞对于脑组织损伤后的修复起着重要作用。

Objective To explore the expression and proliferation of neural stem cell induced f ollowing brain injury of mature rat.Methods The cortex and caudate nucleus of mature SD rats were cut transversely in s tereotaxic apparatus and animals we re killed in 1week,2weeks,3weeks and 4weeks following injury.The reaction pattern o f neural stem cell after brain injury was observed by Nestin im-munostaining and Nissl staining.Results There were a lot of neural stem cells a ppear in the subven tricular zone(SVZ)at the damaged side,the oppose side and the third brain ve ntricle,especially on about 1week.The expression and proliferation of neural stem cells could also be found around the wound,especially i n about 2weeks.The expression and pr oliferation of neural stem cells showed a gradient pattern around the wound.Conclusion The damage of brain can induce the expression and proli feration of neural stem cells around SVZ and wound and neural stem cells play a chief role in repair ing.