纳洛酮对大鼠脑外伤后神经细胞凋亡的影响

The effect of naloxone on rat neural cells apoptosis after traumatic brain injury

目的 :采用Feeney法自由落体撞击脑损伤动物模型 ,观察纳洛酮对脑外伤后神经细胞凋亡和脑水肿的影响。方法 :90只大鼠分为实验组及对照组 ,在损伤后 15、6 0min及 2 4h分别给予纳洛酮或生理盐水 ,损伤后第 5d断头处死大鼠 ,TUNEL法测定神经细胞原位凋亡情况 ,测定损伤侧大脑半球含水量以及光镜下观察细胞形态学改变。结果 :与对照组比较 ,大鼠脑外伤后 15及 6 0min静注纳洛酮可保护神经细胞 ,减少凋亡率 ,减轻脑水肿 ;而外伤后 2 4h给药 ,神经细胞凋亡率以及脑水肿无显著性差异。结论 :研究结果提示纳洛酮作为阿片受体的拮抗剂有保护大鼠脑外伤后神经细胞的作用 ,但应在外伤后尽早用药。

Aim: To investigate the effect of Naloxone on the rat neural cells apoptosis induced by brain trauma. Methods: Animal model was established with the free-falling-body-crash-device of Feeney. Ninety SD rats were divided into control-group and trial-group, Naloxone or saline was injected in two groups separately at minute 15, minute 60 and hour 24 after brain injury, respectively. Five days after the injury, cerebral water content was evaluated; cell morphology was observed under light microscope; neural cells apoptosis was valued by TUNEL in situ cell death kit. Results: Compared to the control-group, Naloxone relieved the neural apoptosis and brain edema induced by brain trauma, when it was injected 15 min and 60 min after the injury. However, there was no obvious difference in apoptosis or brain edema, when naloxone was used 24 hours after the injury. Conclusion: An early large dosage of Naloxone, as an opium receptor antagonist, can relieve traumatic brain edema and protect neural cells from apoptosis.