人类疱疹病毒7U41蛋白DNA链结合能力分析

Identification of the DNA binding ability of human herpesvirus 7 U41 protein

目的 :对人类疱疹病毒 7(humanherpesvirus 7,HHV - 7)U4 1的核酸结合能力进行分析鉴定。方法 :DNA结合蛋白分离收集、免疫沉淀及Western印迹检测U4 1蛋白对单、双链DNA的不同结合能力。结果 :U4 1蛋白能结合单链DNA而不能结合双链DNA。结论 :U4 1的功能之一是保持DNA模板的合适构型 ,以便DNA的延伸和病毒DNA的合成。

Aim: To identify the DNA binding ability of human herpesvirus 7(HHV-7) U41 protein. Methods: DNA binding assay and Western blot were done to detect the single and double DNA strands binding ability of U41 protein. Results: the U41 protein was able to bind to a single-strand DNA cellulose column but not to double-strand DNA cellulose column. Conclusion: These findings strongly suggest that the major function of U41 is to keep the DNA template in the optimal conformation for DNA elongation and be required for viral DNA synthesis.