女性激素和AgNOR在胆囊癌中表达的研究

Studied on expression of female hormone and AgNOR in gallbladder carcinoma

目的 :研究雌激素受体 (ER)、孕激素受体 (PR)在 40例胆囊癌中表达和AgNOR在胆囊癌中含量及形态变化的关系。方法 :用免疫组化ABC法和AgNOR技术对胆囊正常上皮和癌上皮进行实验测定。结果 :胆囊正常上皮ER、PR为阴性 ,癌上皮ER2 6例 (6 5 %)阳性 ,PR2 0例 (5 0 %)阳性 ,核仁组成区相关蛋白 (AgNOR)表示颗粒类型 ,正常上皮为单一型 ,癌上皮主要表现为弥散型 ,其次为混合型。结论 :胆囊癌的发生发展对女性激素有一定的依赖性 ,AgNOR计数及形态分类对胆囊癌的生物学行为、预后、病理诊断及治疗均有一定的参考价值。

Objective:The purpose was to study the relationship between estrogen receptor(ER),progesterone receptor(PR),expression and the content,pattern changes of Argyrophilic Nucleolar Organizer Regions(AgNOR)in 40 cases of gallbladder carcinoma.Methods:An immunohistochemical method utilizing avidin complex(ABC)and AgNOR technique were used in this study to detect the expressions in the normal and carcinous epithelia in gallbladder.Results:ER,PR were negative in the normal epithelia.The positive staining for ER was observed in 26 cases of gallbladder carcinoma(65%),for PR was in 20 cases(50%).AgNOR which was expressed as granular pattern were single type in normal epithelia,but in major of carcinous epithelia expressed as diffusive type,secondly as mixed type.Conclusion:The occurrence and development of gallbladder carcinoma was somewhat dependent on female hormone.The counts and classification in structure of AgNOR had significant value in the evaluation of biological behavior,prognosis,pathologic diagnosis and treatment of gallbladder carcinoma.