蛛网膜下腔出血与皮质神经元凋亡

Apoptosis of Cortical Neurons after Subarachnoid Hemorrhage in Rabbits

目的 探讨蛛网膜下腔出血(SAH)对皮质神经元的损伤及其机制,为临床脑保护提供有效的治疗方法。方法 将40只大耳白兔随机分为5组:A组(空白对照组)、B组、C组、D组、E组(实验组)。脑池内血液注入法制作SAH模型。对A组动物脑枕大池内注入生理盐水,对B、C、D、E组动物注入自体鲜血。分别于术后1d(B组)、3d(C组)、5d(D组)及7d(E组)处死动物,取颞叶的大脑皮质行光镜观察及用凋亡细胞原位末端标记技术对神经细胞进行检测。结果 实验组动物均可检测出凋亡细胞,B组神经细胞凋亡指数低于C组、D组、E组(P<0.05)。结论 SAH后出现迟发性神经元死亡(DND),凋亡在DND中起重要的作用。

Objective To explore the mechanism of the damage to cortical neurons and effective therapy for the brain protection after subarachnoid hemorrhage (SAH) in the rabbits. Methods Forty white rabbits were randomly divided into five groups i.e. group A (blank control), B, C, D and E. Fresh autologous blood was injected into the cisterna magna to establish the model of SAH in the rabbits of groups B, C, D and E, and normal saline was injected into the cisterna magna in the rabbits of group A. The animals of group B, C, D and E were sacrificied respectively on day 1, 3, 5 and 7 after the injection. The animals of group A were simultaneously killed. Apoptosis cells were detected by the terminal deoxynuleotidyl transferase -mediated dUPT -biotin nick end labeling and light microscope. Results Apoptosis cells were detectal in all the experimental groups. Apoptosis labeling Index (AI) in group B was significantly lower than that in group C, D and E (P<0.05). Conclusion There is some delayed neuronal death (DND) after SAH in the rabbits. Apoptosis plays an important role in DND.