四川大头茶过氧化物酶和细胞色素氧化酶同工酶变异的数量分析

The Numerical Analysis of Isozymic Variation of POD and CYT inGordonia acuminata

运用同工酶电泳技术 ,测定了来自不同地理种源和不同演替群落的四川大头茶 (Gordoniaacuminata (pritz)Chang) 8个居群 112株个体功能叶片的过氧化物酶 (POD) ,细胞色素氧化酶 (CYT)的同工酶谱带 ,并进行编码 ;然后采用Rogers-Tanimoto结合系数的组平均法 (UPGMA)对各酶谱带进行聚类分析 ;结果表明 :来自不同演替群落的四川大头茶居群及个体间存在着极明显的同工酶变异和遗传分化 ,来自相同演替群落的居群及个体间存在着极大的同工酶谱相似性和遗传相似性 ;环境因子在四川大头茶居群遗传分化过程中起着非常重要的作用 ;但不同的环境因子在不同居群的同工酶谱和遗传分化中所起的作用不同 ;群落演替类型对四川大头茶的同工酶谱和遗传分化起着重要作用的同时 。

The peroxidase (POD) and cytochromeoxidase (CYT) isozymic variation in seedling leaves of 112 individuals from 8 isolated G. accuminata populations, was studied with electrophoretic technique. The isozymic bands of two enzymes were coded to construct the two state data matrix which was calculated with the Rogers Tanimoto association coefficients clustered by UPGMA. Results indicated thatindividuals from the same population showed higher similarity in isozymic bands and higher genetic homogeneity, while individuals from different populations had high genetic dissimilarities. The effects of provenance on isozymic band patterns and genetic differentiation follows the following sequence populations from different geographical area,populations from different type of communities, populations and individuals from same communities, different micro populations in same populations, individuals within a micro-population. It is likely that the isozymic variation of POD and CYT are affected by the distributive patterns and reproductive features of Gordonia acuminata, as well as environmental factors, soil pH, altitude, soil types, and soil organotrophes.